Fig. 3.

In vivo BBB model. Mice were inoculated with PBS or 100 PFU of WNV via footpad and BBB permeability was measured using Evans blue dye at day 6 after infection. Mice were injected i.p. with 1 mL Evans blue dye (1 % w/v) and after 1 h were cardiac perfused with PBS. The extravasation of the dye was evident in the WNV-infected mice brain