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. Author manuscript; available in PMC: 2018 Jun 2.
Published in final edited form as: Thromb Haemost. 2017 Apr 27;117(6):1150–1163. doi: 10.1160/TH16-05-0354

Figure 2. Del-1–deficiency affects angiogenesis in a non-endothelial cell autonomous way.

Figure 2

(A and B) Three-dimensional in vitro angiogenic sprouting in a spheroidal culture system with collagen–embedded spheroids is shown. (A) HUVEC were transfected with siRNAs targeting Del-1 or control siRNA. Three-dimensional in vitro angiogenic sprouting in a spheroidal culture system with collagen–embedded spheroids in the absence (control) or presence of bFGF (30 ng/mL). The mean cumulative length of sprouts per spheroid was assessed after 24 h. Silencing of endogenous Del-1 did not significantly affect angiogenic sprouting (n=3). (B) Representative images of spheroidal sprouting assay performed with endothelial cells transfected as indicated with control siRNA or Del-1 siRNA in the presence of bFGF (30 ng/mL). (C) Sprouting of aortic rings from WT and Del-1−/− mice in the absence (control) or presence of VEGF was performed. The number of angiogenic sprouts per aortic ring was assessed by confocal microscopy (*P<0.05 vs WT control and #P<0.05 vs Del-1−/− control; n.s.: not significant; n= 21–28 aortic rings per group). (D) Representative images of aortic rings from a WT and a Del-1−/− mouse.