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. Author manuscript; available in PMC: 2018 Jun 2.

Published in final edited form as: Thromb Haemost. 2017 Apr 27;117(6):1150–1163. doi: 10.1160/TH16-05-0354

(A) Infiltration of ischemic retinas on postnatal day 15 with CD45⁺ leukocytes in WT and Del-1^−/− mice in the ROP model is shown. Quantification of CD45⁺ leukocytes in ischemic retinas by fluorescence microscopy in WT and Del-1–deficient mice (*P<0.05 vs. WT, n=4). (B) Infiltration of ischemic muscles with CD45⁺ leukocytes (green fluorescence) in WT and Del-1^−/− mice 2 weeks after the induction of hind limb ischemia, as assessed by confocal microscopy (40X objective). The red fluorescence indicates PECAM-1⁺ vessels. (C) Flow cytometry of digested muscles 4 days after induction of hind limb ischemia is shown. The infiltrated leukocytes were analysed by flow cytometry for leukocyte subset markets. Data are presented as infiltrated cells/mg tissue ± SEM. (*P<0.05 vs ischemic Del-1^+/+, n=5–7/group).

(A) Infiltration of ischemic retinas on postnatal day 15 with CD45⁺ leukocytes in WT and Del-1^−/− mice in the ROP model is shown. Quantification of CD45⁺ leukocytes in ischemic retinas by fluorescence microscopy in WT and Del-1–deficient mice (*P<0.05 vs. WT, n=4). (B) Infiltration of ischemic muscles with CD45⁺ leukocytes (green fluorescence) in WT and Del-1^−/− mice 2 weeks after the induction of hind limb ischemia, as assessed by confocal microscopy (40X objective). The red fluorescence indicates PECAM-1⁺ vessels. (C) Flow cytometry of digested muscles 4 days after induction of hind limb ischemia is shown. The infiltrated leukocytes were analysed by flow cytometry for leukocyte subset markets. Data are presented as infiltrated cells/mg tissue ± SEM. (*P<0.05 vs ischemic Del-1^+/+, n=5–7/group).