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. Author manuscript; available in PMC: 2018 Jun 2.
Published in final edited form as: Thromb Haemost. 2017 Apr 27;117(6):1150–1163. doi: 10.1160/TH16-05-0354

Figure 4. Del-1 inhibits adhesion of leukocytes on endothelial cell monolayers.

Figure 4

(A) Binding of PMA-stimulated human MNC to immobilized Del-1 in the presence of isotype control antibody or neutralizing monoclonal antibodies against β2-integrins: against αMβ2-integrin (Mac1) (clone: CBRM1/5) or against αLβ2-integrin (LFA-1) (clone 38). Data are presented as adhesion % of cell input ± SEM (*P<0.05 vs. PMA+ control IgG, n=6). (B) Adhesion of human MNC cells onto TNF-stimulated or non-stimulated HUVEC monolayers 48 h after transfection of HUVEC with control siRNA or Del-1 siRNA. Data are presented as adhesion % of cell input ± SEM (*P<0.05 vs. control siRNA+TNF; n=4). (C) Cell tracker Red-labelled bone marrow–derived Lin- hematopoietic progenitor cells were intravenously injected in WT or Del-1−/− mice one day after the induction of hind limb ischemia. The ischemic muscles were harvested 24h thereafter. Quantification of the infiltration of ischemic muscles with Cell tracker Red-labelled bone marrow–derived Lin- hematopoietic progenitor cells. Data are presented as infiltrated Lin- in % of WT ± SEM (*P<0.05 vs. WT; n=3/group).