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. Author manuscript; available in PMC: 2017 Jul 9.
Published in final edited form as: Science. 2017 Mar 10;355(6329):1072–1076. doi: 10.1126/science.aak9748

Fig. 2. c-Fos and GRP expression in the suprachiasmatic nucleus of mice with contagious itch.

Fig. 2

(A) Heat map showing comparison of normalized fold change in number of c-Fos+ neurons in select brain regions between mice observing ambulating (control) or scratching demonstrators. (B) Schematic of coronal brain section with suprachiasmatic nucleus (SCN) enlarged with core and shell regions, third ventricle (3V), and optic chiasm (OC) indicated. (C and D) c-Fos expression in SCN of mice observing control (C) and scratching (D) demonstrators. (E) Mean number of c-Fos neurons in SCN of control and scratching observer groups. (F and G) GRP immunohistochemistry in SCN of mice observing control (F) and scratching (G) videos. (H) Mean normalized intensity of GRP staining in SCN sections of control and scratching observer groups. (I) Image of Grp in situ hybridization (ISH) in SCN. (J) Higher-magnification image of Grp in situ hybridization in the shell and core, respectively. (K and L) AVP immunohistochemistry image in SCN of mice observing control (K) and scratching (L) demonstrators. (M) Mean normalized intensity of AVP staining in SCN sections of control and scratching observer groups. Scale bars, 100 µm [(C), (D), (F), (G), (I), (K), and (L)], 20 µm (J). n = 3 mice and 10 sections per group. Unpaired t test in (E), (H), and (M); ***P < 0.001. Data are means ± SEM.