Table 2.
Correlations of substrate length, 2nd structure, and charge ratio to the assembly products.
| Substrate | Charge ratio for T=1 | Product |
|---|---|---|
|
| ||
| 300 nt ssRNA | 1 | T=1 |
| 500 nt ssRNA | 1.67 | T=1 |
| 600 nt ssRNA | 2 | T=1 |
| 800 nt ssRNA | 2.67 | T=1 and oversized particles |
| 600 mer PSS | 2* | Doublets, triplets, and T=1 |
For PSS reactions where the initial ratio of pentamers per polymer was 12:1, doublets and triplets comprised of one PSS and 24 or 36 pentamers would be neutral or undercharged with ratios of 1.0 and 0.67, respectively. Therefore, we cannot exclude the possibility that they contain more than one PSS molecule per complex.