FIGURE 4.
Plant growth inhibition by UV-B in haf1 plants. WT (Ws and Col-0) and haf1-3 and haf1-4 plants were treated with UV-B radiation for 4 h (9 μmol m-2 s-1, right) or were kept under conditions in the absence of UV-B. (A,C) Rosette area of control and UV-B treated Col-0 and haf1-3 (A), and Ws and haf1-4 (C) plants measured every 3 days from germination until 21 DAS. Plants were UV-B treated 12 DAS (indicated with an arrow). (B,D) The ratio of rosette areas of UV-B treated vs control plants for each line is shown. Results represent the average of 10 biological replicates ±SEM. Different letters denote statistical differences applying Student’s t-test (P < 0.05). (E–G) UV-B effect in leaf development in haf1-3 plants. Relative average leaf area (E), estimated cell number (F) and cell area (G) of fully expanded leaf #5 from UV-B treated versus control Col-0 and haf1-3 Arabidopsis plants. Results represent the average of 10 biological replicates ±SEM. Statistical significance was analyzed using ANOVA, Tukey test with P < 0.05; differences from the control are marked with different letters.