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. 2017 Jul 10;8:1179. doi: 10.3389/fpls.2017.01179

FIGURE 7.

FIGURE 7

UV-B effect in flowering time in WT, hac1, hac5, and hac12 mutants. Days before flowering (A) and number of leaves at flowering time (B) for WT, hac1-1, hac5-7, and hac12-2 mutants under control conditions, or in plants that were grown in the absence of UV-B for 9 days, and they were then irradiated with UV-B for 1 h/day until flowering, at an intensity of 9 μmol m-2 s-1 under long day photoperiod. (C,D) The ratio of flowering time, analyzed by counting the days before flowering (C) and the number of leaves at flowering time (D) of UV-B treated vs control plants for each line is shown. Results represent the average of 10 biological replicates ±SEM. (E,F) Relative expression of FLC (E) and SOC1 (F) transcripts in Col-0 and hac1-1 plants by RT-qPCR. Plants were irradiated with UV-B for 4 h (UV-B) or were kept under control conditions without UV-B (C). Expression values are relative to the CPK3 control. Data show mean values ±SEM of at least three independent experiments. Statistical significance was analyzed using ANOVA, Tukey test with P < 0.05; differences from the control are marked with different letters. (G) ChIP-qPCR analysis of Ac H3 and total H3 associated to the promoter region of FLC in UV-B irradiated and control Col-0 and hac1-1 plants. Data was normalized to values obtained using input DNA before immunoprecipitation Results represent the average of three biological replicates ±SEM. Statistical significance was analyzed using ANOVA, Tukey test with P < 0.05; differences from the control are marked with different letters.