Figure 5.

Direct Interaction of ARR1 with the WUS Promoter Region.

(A) and (B) Phylogenetic footprinting analysis of the WUS genomic region. Black bold lines with a to e indicate fragments amplified by ChIP-qPCR, and white boxes represent the GAT(T/C) in (A), with adjacent sequences shown in (B). Green, adenine; red, thymine; blue, cytosine; purple, guanine; yellow, conserved GAT(T/C) domain highlight.

(C) ChIP of ARR1ΔDDK-MYC protein with WUS chromatin regions. Shoot tissues (with leaves removed) were used.

(D) EMSA of ARRM-GST with the WUS genomic regions. ARRM indicates the DNA binding domain of ARR1.

(E) to (G) Ratio of firefly luciferase (Luc) to Renilla luciferase (Ren) activity in Arabidopsis protoplasts cotransformed with different reporter and effector construct combinations. Error bars in (C) to (G) indicate the sd of three biological replicates run in triplicate. *P < 0.05 and **P < 0.01 (Student’s t test).