Figure 1.

General Experimental Approach.

The general experimental approach performed in this study is shown, and three sections are highlighted: poly(A) tag library preparation (left), sequencing data workflow (middle), and downstream analyses (right), including gene expression analysis, distribution of PATs, relative poly(A) site usage, and Gene Ontology enrichment. In the right panel, the relative poly(A) usage was calculated as the ratio of the fractional contribution of individual clusters to total poly(A) usage for a gene after treatment divided by the fractional contribution in control conditions; an example for relative poly(A) usage in the coding region (labeled as C in the equation) is shown.