Figure 4.

CO Binds the FT Promoter in an At-NF-YB2-NF-YB3-Dependent Manner.

ChIP was performed on Col-0 (parental) and nf-yb2 nf-yb3 plants transgenic for p35S:CO-YFP/HA. Enrichment of the selected segments -CCAAT/-5.3kb, core promoter, exon 4/+2.0kb- were evaluated by qPCR with appropriate amplicons. Error bars indicate se with five biological replicates. In each replicate, three technical replicates were performed. Statistical significance was obtained using Bio-Rad CFX Manager Version 3.0; in each case, the comparison is between the nonimmune control (NIC) and the immunoprecipitation (IP). ***P < 0.001.