Figure 1.

miR159 Regulates Vegetative Phase Change in Arabidopsis by Affecting miR156 and SPLs.

(A) Twenty-one-day-old Col-0, mir159ab, and UBI10:miR159a plants grown in short days. Bar = 1 cm.

(B) Leaf shape and abaxial trichome phenotypes of fully expanded rosette leaves of Col-0, mir159ab, and UBI10:miR159a in short days. Numbers indicate the first leaf with abaxial trichomes. Different capital letters indicate significant difference between genotypes using one-way ANOVA at P < 0.01 (n = 30 plants, ±sd; Supplemental File 1). Bar = 1 cm.

(C) Leaf initiation rate of Col-0, mir159ab, and UBI10:miR159a in short days. Leaf numbers were scored at 8, 12, 16, 20, 24, and 28 d after planting. Asterisks indicate significant difference from Col-0 using Student’s t test (P < 0.01, n = 30 plants, ±sd).

(D) to (F) The expression of miR156 (D), Pri-MIR156A (E), and SPL9 (F) at different developmental stages in Col-0 and mir159ab plants assessed using qRT-PCR. About 2-mm shoot apices were collected at 10, 14, 18, and 22 d after planting in short days. Asterisks indicate significant difference from Col-0 using Student’s t test (P < 0.01).

(G) The expression of different SPL genes in 10-d-old Col-0 and mir159ab plants assessed using qRT-PCR. About 2-mm shoot apices were collected at 10 d after planting in short days. Asterisks indicate significant difference from Col-0 using Student’s t test (P < 0.01).

All qRT-PCR data ([D] to [G]) represent the mean of three biological replicates (experiments were repeated at different times, the same for the following figures); values were normalized to the 10-d-old wild type (±sd).