Figure 5.

Association of Ku Complexes with Telomeres and Their Ability to Protect DNA Ends.

(A) Association of Ku with telomeres assessed by ChIP with Ku70 and histone H3 antibodies. Control reaction with no antibody was run in parallel. Error bars represent sd from three technical replications. Increased amount of telomeric DNA immunoprecipitated by histone H3 antibody in At25 and KU70-null plants reflects longer telomeres in these mutants.

(B) Diagram depicting individual steps of the nuclease protection assay coupled to PIFE.

(C) Association of Ku with Cy3-labeled 25-bp DNA determined by PIFE.

(D) Nuclease protection measured as the intensity of Cy3 signal after T7exo treatment relative to DNA probe without added protein. Data in (C) and (D) are derived from consecutive measurements of the same samples. Error bars indicate sd from at least three replicas.