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. 2017 May 30;29(6):1460–1479. doi: 10.1105/tpc.17.00136

Figure 4.

Figure 4.

[Ca2+]cyt Elevations Are Not Detected in the Phloem around Aphid-Feeding Sites.

(A) Left: Normalized fluorescence (∆F/F) around aphid-feeding sites of 35S:GCaMP3 aphid-exposed leaves and no-aphid controls. Error bars represent se of the mean (n = 31). Gray shading indicates significant difference between treatments (Student’s t test within GLM at P < 0.05). Right: Representative stereomicroscope image of a [Ca2+]cyt elevation seen around an aphid-feeding site on a 35S:GCaMP3 leaf. GFP fluorescence is color-coded according to the inset scale. Aphid outlined in yellow and feeding site indicated with an arrowhead. Image taken at 3 min after settling.

(B) Left: ∆F/F around the aphid-feeding sites of SUC2pro:GCaMP3 aphid-exposed leaves and no-aphid controls. Error bars represent se of the mean (n = 34). Right: Representative stereomicroscope image of the absence of [Ca2+]cyt elevation around an aphid-feeding site on a SUC2pro:GCaMP3 leaf. GFP fluorescence is color-coded according to the inset scale. Aphid outlined in yellow and feeding site indicated with an arrowhead. Image taken at 3 min after settling.