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. 2017 May 30;29(6):1460–1479. doi: 10.1105/tpc.17.00136

Figure 8.

Figure 8.

TPC1 Overexpression Has No Effect on Aphid-Elicited [Ca2+]cyt Elevations.

(A) Left: Normalized fluorescence (∆F/F) around feeding sites of aphid-exposed 35S:GCaMP3 leaves and no-aphid controls. Error bars represent se of the mean (n = 30). Gray shading indicates significant difference between treatments (Student’s t test within GLM at P < 0.05). Right: Representative stereomicroscope image of the [Ca2+]cyt elevation seen around a feeding site of a 35S:GCaMP3 leaf. GFP fluorescence is color-coded according to the inset scale. Aphid outlined in yellow and feeding site indicated with an arrowhead. Image taken 6 min after settling.

(B) Left: ∆F/F around feeding sites of aphid-exposed 35S:GCaMP3 × 35S:TPC1 5.6 leaves and no-aphid controls. Error bars represent se of the mean (n = 29). Gray shaded areas indicate significant differences between the two treatments (Student’s t test within GLM at P < 0.05). Right: Representative stereomicroscope of the [Ca2+]cyt elevation seen around a feeding site of a 35S:GCaMP3 × 35S:TPC1 5.6 leaf. GFP fluorescence is color-coded according to the inset scale. Aphid outlined in yellow and feeding site indicated with an arrowhead. Image taken 5 min after settling.

(C) Comparison of ∆F/F around feeding sites of aphid-exposed 35S:GCaMP3 and 35S:GCaMP3 × 35S:TPC1 5.6 leaves. Data of aphid exposures shown in (A) and (B) were replotted together. Areas shaded in gray indicate significant differences between the two treatments (Student’s t test within GLM at P < 0.05).