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. 2017 May 30;29(6):1460–1479. doi: 10.1105/tpc.17.00136

Figure 9.

Figure 9.

TPC1 Overactivation (in the fou2 Mutant) Results in Systemic [Ca2+]cyt Elevations in Response to Aphid Feeding.

(A) Comparison of normalized fluorescence (∆F/F) around feeding sites of aphid-exposed 35S:GCaMP3 and aphid-exposed 35S:GCaMP3 × fou2 leaves. Error bars represent se of the mean (35S:GCaMP3 n = 28; 35S:GCaMP3 × fou2 n = 25).

(B) Comparison of ∆F/F around systemic lateral tissue sites of aphid-exposed 35S:GCaMP3 and aphid-exposed 35S:GCaMP3 x fou2 leaves. Error bars represent se of the mean (35S:GCaMP3 n = 28; 35S:GCaMP3 × fou2 n = 25). Gray shading indicates significant difference between treatments (Student’s t test within GLM at P < 0.05).

(C) Representative stereomicroscope images of the [Ca2+]cyt elevations seen in 35S:GCaMP3 (left) 35S:GCaMP3 × fou2 (right) leaves exposed to M. persicae. GFP fluorescence is color-coded according to the inset scale. Aphid outlined in yellow and feeding site indicated with an arrowhead. Image on left taken at 2 min after settling; image on right taken at 10 min after settling.