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. 2017 Jun 2;29(6):1425–1439. doi: 10.1105/tpc.17.00364

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Figure 3. — WRKY46, WRKY54, and WRKY70 Directly Interact with BES1 Both in Vivo and in Vitro.

(A) WRKY46/54/70 interact with BES1 by BiFC assay in vivo. Cotransformation of WRKY46/54/70-YFPC and BES1-YFPN led to the reconstitution of YFP signal, whereas no signal was detected when BES1-YFPN and YFPC or WRKY46/54/70-YFPC and YFPN were coexpressed (Supplemental Figure 7F). The experiments were performed twice with similar results.

(B) and (C) Transient expression of LUC driven by the BR-regulated gene promoters of At2g45210 (B) and At1g43910 (C). The mean and sd were derived from three biological repeats.