| Dilution | Amount of cDNA | Amount of H2O (μl) | Equivalent full-scale PCR cycles (step 105) |
|---|---|---|---|
| 1 | 2 μl RT sample | 6 | 17 |
| 2 | 2 μl dilution 1 | 6 | 15 |
| 3 | 2 μl dilution 2 | 6 | 13 |
| 4 | 2 μl dilution 3 | 6 | 11 |
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