| Reagents | Volume per reaction (ml) | Final concentration |
|---|---|---|
| T4 RNA ligase buffer (10x) | 1 | 1x |
| ATP (10 mM) | 1 | 1 mM |
| 50% PEG | 2 | 10 % |
| RNase inhibitor | 1 | 4 units per ml |
| T4 RNA ligase I | 1 | 1 units per ml |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.