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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Nutr Biochem. 2017 Apr 12;45:94–103. doi: 10.1016/j.jnutbio.2017.04.004

Figure 5. Mice consuming a high fat diet have impaired liver complex I to III enzyme activity.

Figure 5

(A) Representative BN-PAGE gel, complexes (C-I-V) and supercomplexes (SC) from mice consuming a lean or high fat diet. “SD” is the molecular weight markers, “C” is lean control, and “H” is high fat diet. (B) Quantification of BN-PAGE. (C) Specific enzymatic activities of individual complexes and (D) NADH oxidation or succinate oxidation coupled to cytochrome c reduction (CI or CII to CIII). Activities were determined relative to total protein content and then normalized to citrate synthase (CS) activity. Data are the averages ± S.E.M. from 4 independent experiments. The asterisk indicates significance from control (*p < 0.05).