Table 1.

KIEs for RNA Depurination Catalyzed by Saporin L3 A14C

RNA 10-mer	type of KIE	experimental KIE	intrinsic KIE	computed KIE
[1′-14C], [5′-3H]	primary 14C	1.051 ± 0.008a	1.052 ± 0.008	1.052
[9-15N, 1′-14C], [5′-3H]	combined 14C + 15N	1.062 ± 0.009a	1.064 ± 0.009	1.073
[1′-3H], [5′-14C]	α-secondary 3H	1.044 ± 0.004	1.045 ± 0.004	1.048
[4′-3H], [5′-14C]	γ-secondary 3H	0.961 ± 0.004	0.960 ± 0.004	0.943
[5′-3H], [5′-14C]	δ-secondary 3H	1.040 ± 0.006	1.041 ± 0.006	1.042
[7-15N, 5′-14C], [5′-3H]	secondary 15N	0.974 ± 0.007a	0.973 ± 0.007	0.984
[9-15N, 5′-14C], [5′-3H]	primary 15N	1.022 ± 0.009a	1.023 ± 0.009	1.022

^aKIEs were corrected for the 5′-³H KIE according to the expression: KIE_experimental = KIE_observed(5′-³H KIE).