Fig. 3.

Graphs of the extent of hydrolysis of the 4-nitrophenoxy silyl ether substrates 1 (A), 2 (B), and 3 (C) against time, as measured by UV-Vis absorbance at 405 nm corresponding to the 4-nitrophenoxylate anion (calibrated using data from SI Appendix, Fig. S4). Assays are conducted with 0.00067 mol eq of enzyme relative to 0.1 mM substrate in 5% 1,4-dioxane, 50 mM Tris, and 100 mM NaCl, pH 8.5 at 22 °C.