Table 2.

Table of percentage conversions for the enzyme catalyzed hydrolysis of the various substrates

Enzyme	Net % conversion
	1	30	31	7
TF-Silα	19.2 ± 1.2	<0.01 ± 0.0	<0.01 ± 0.0	<0.01 ± 0.0
Silα	21.4 ± 0.3	1.3 ± 0.8	<0.01 ± 0.0	<0.01 ± 0.0
Chymotrypsin	6.9 ± 0.05	17.5 ± 7.1	<0.01 ± 0.0	21.4 ± 0.5
Cathepsin L*	2.5 ± 1.3	1.6 ± 1.7	<0.2 ± 0.01	26.7 ± 1.8

Net conversions are calculated after subtraction of background hydrolysis. All reactions were performed for 4 h with 0.00067 mol eq enzyme with 0.2 mM substrate in 5% vol/vol dioxane, 50 mM Tris, and 100 mM NaCl.

^*Assay was conducted at pH 6.8, the optimum for this enzyme; all other assays were performed at pH 8.5.