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MALDI-TOF spectra of oxidized and reduced mambaquaretin-1. Shown are MALDI-TOF spectra of mambaquaretin-1 before (Top) and after (Bottom) reduction of the disulfide bonds. Each reduced disulfide bond leads to the molecular weight increase by 2 Da. The difference between the masses of the two species charged with one proton clearly indicates that mambaquaretin-1 has three disulfide bonds (M = 6,374.23 to 6,368.21 = 6.02 Da). Monoisotopic masses are in bold.
