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. 2017 Jun 5;114(27):7083–7088. doi: 10.1073/pnas.1620835114

Fig. 3.

Fig. 3.

IL-2 effectively promotes suppressive Treg survival in the presence of CNIs. Whole blood was cultured for 4 d in the presence of physiological concentrations of tacrolimus (TAC) (5–25 ng/mL) with or without daily addition of IL-2 (10 and 100 IU/mL) or IL-7 (2.5 and 25 pg/mL). (A) Quantification of CD4+FOXP3+ T cells before (D0) and after 4-d culture of whole blood from healthy controls (HC) and from liver transplant patients (TX) treated with IL-2 (n = 6) or IL-7 (n = 10) or without IL-2 or IL-7 treatment (CN). (B) Quantification of Treg subsets based on CD45RA and FOXP3 expression in liver transplant patients after 4 d of culture in the indicated conditions. (C) Flow cytometry analysis of Annexin V and BCL2 in Treg subsets of liver transplant recipients after 4 d of culture. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.