Fig. 1.

The multiplexed detection of CV-specific IgG autoantibodies on plasmonic gold chips using an ultralow volume of serum samples. Schematic depicting the plasmonic nano-gold islands deposited on glass slide surface, the sandwich microarray structure, and the actual assay steps including (i) the immobilization of an array of CV antigens on the chip, (ii) the capture of the targeted autoantibodies from diluted patients’ serum, and (iii) the binding of secondary detection antibodies conjugated with IRDye 800 fluorescent dye for signal reporting.