Fig. 1.

Development of PDAC in the KPB mice. (A) Kaplan-Meier analysis for comparing overall survival of the WT, KP, and KPB mice. The median survival of the KP and KPB mice is 157 and 62 d, respectively (P < 0.001, log-rank test, for each pairwise combination). (B) Anatomic photograph of KPB mouse with PDAC. Outline denotes the tumor. Note that the distensible gallbladder (marked by *) is a common symptom accompanied with PDAC. Gb, gallbladder; L, liver; Sp: spleen; T, tumor. (C) Histomorphology of normal pancreas in the WT mice and different stages of PanINs and invasive PDAC in the KPB mice. Arrowheads indicate the representative morphology in different stages of PanINs. (Scale bars, 50 µm.) (D) Immunostaining of γH2AX and 53BP1 in pancreatic tissues of the WT mice, and precancerous or cancerous pancreases of the KP and KPB mice. (Scale bars, 50 µm.) (E) Representative mitotic spreads of the WT and KPB pancreatic cell. Chromosomes were stained with Giemsa. Chromosome breaks from pancreatic tumors of the KPB mice are shown. Sixteen mitotic spreads in each sample were examined. Percentage of chromosome breaks in each sample is summarized. Means and SDs were plotted. ***P < 0.001.