Table 1.
Michaelis–Menten kinetic parameters for cysteine turnover by Fe–S cluster assembly complexes
| Complex | KM, µM | kcat, min−1 | kcat/KM, M−1⋅s−1 | Source |
| NFS1–ISD11 (SD) | 340 ± 60 | 1.9 ± 0.1 | 93 ± 20 | 25 |
| SD + FXN (SDF) | 330 ± 60 | 1.7 ± 0.1 | 86 ± 20 | 25 |
| SD + ISCU2 (SDU) | 590 ± 50 | 0.89 ± 0.04 | 25 ± 2 | 25 |
| SDAec | 1.3 ± 0.3 | 0.60 ± 0.04 | 8,000 ± 2,000 | This work |
| SDAec + ISCU2 (SDAecU) | 0.82 ± 0.3 | 0.62 ± 0.11 | 13,000 ± 5,000 | This work |
| SDUF | 11 ± 3 | 5.2 ± 0.4 | 7,900 ± 2,000 | 25 |
| SDAecUF | 10.1 ± 0.2 | 11.6 ± 0.9 | 19,100 ± 2,000 | This work |
| IscS | 17 ± 2 | 7.5 ± 0.1 | 7,400 ± 900 | 49 |