Fig. S1.

CD4 and CD8 T-cell response in MHV-infected brains of WT and DP1^−/− mice. Brains from MHV-infected WT or DP1^−/− mice were harvested at 8 dpi for quantifying T-cell responses. (A) Brain cells were incubated with M133 peptide in the presence of Brefeldin A for 6 h and were assayed for IFN and TNF using flow cytometric analysis. The frequency and number of IFN ⁺ cells (Left and Center) and the frequency of TNF⁺IFN ⁺ cells (Right) (as a measure of polyfunctionality) are shown. (B) The frequency (Left) and total number (Right) of FoxP3⁺ CD4 T cells are shown. (C) The frequency (Left) and total number (Right) of virus-specific CD8 T cells, determined using MHC class I/peptide S510 tetramers are shown. Data represent the mean ± SEM of two independent experiments; n = 4 mice per group; *P < 0.05; **P < 0.01; ***P < 0.001, Mann–Whitney U test; n.s., not significant.