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The line width versus fraction bound of the proton peaks of THB. The line width of H1 (A), H2 (B), H6 (C), and H7 (D) proton peaks as a function of fraction THB bound. Conditions: THB (150, 300, 600, and 1,200 µM), spin-labeled SULT1A3 [control (black), 27 (green), 151 (blue), and 234 (red), 30 µM monomer], PAP (500 µM, 17 × Kd), deuterated DTT (5.0 mM), KPO4 (50 mM), pH 7.4, 25 ± 1 °C. The enzyme is >99% saturated at all THB concentrations (Kd THB = 25 nM).
