Table 1.
Enzyme | kcat, min−1 | Km, nM | Ki THB, nM |
WT | 120 (17) | 22 (3.1) | 23 (1.5) |
26* | 110 (15) | 25 (3.5) | 25 (2.7) |
151 | 115 (16) | 26 (2.2) | 20 (2.2) |
234 | 109 (15) | 24 (3.2) | 22 (1.6) |
Values in parentheses indicate SD.
Cys residue at which spin label is attached.