Skip to main content
. 2017 Jun 19;114(27):7095–7100. doi: 10.1073/pnas.1704706114

Fig. 2.

Fig. 2.

PIK3R1 knockdown increases RTK-mediated PI3K/AKT signaling in and transformation of HMECs. (A) Control and shPIK3R1 HMECs were starved and then stimulated with EGF before immunoblotting. (B) Cell suspensions of control and shPIK3R1 HMECs were grown in agar. (C) Control, shPIK3R1, and rescue HMECs were treated as in A before immunoblotting. (D) Cell suspensions of control, shPIK3R1, and PIK3R1 rescue HMECs were grown in agar. (E) Control, shPIK3R1, or p110α-H1047R HMECs expressing activated HER2/neu were starved overnight before immunoblotting. (F) Control and shPIK3R1 HER2/neu HMECs were treated as in A before immunoblotting. (G) Cell suspensions of control and shPIK3R1 HER2/neu HMECs were grown in agar. Means ± SEM are shown; n = 3. *P < 0.05, **P < 0.01, ****P < 0.0001; unpaired t test.