Fig. 4.
Biochemical analysis of AALs and NRPSs. (A) Substrate specificity of ScoA, MmaA, and ScoC determined by ATP-[32P]PPi exchange assays. (B) Detection of crotonyl-S-ScoB and decenoyl-S-MmaB by MS with maximum entropy deconvolution. (C) Extracted ion chromatograms showing the production of LP 3 and 4 in ScoA-C assays using butyric acid and hexanoic acid as a substrate, respectively. (D) Extracted ion chromatogram showing the production of LP 5 in the MmaA-C assay using decanoic acid as a substrate. The calculated masses for LP 3–5 with 10-ppm mass error tolerance were used for each trace.