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. 2017 May 22;114(27):7148–7153. doi: 10.1073/pnas.1705240114

Fig. 5.

Fig. 5.

Annexin A2 facilitates binding of Vamp8 to Stx17. (A) Confocal images showing colocalization of Stx17 (green) with LC3+ (red) vesicles when autophagy is induced by starvation. Arrows indicate the site of colocalization. (Scale bar, 5 μm.) (B) Immunoprecipitates from whole-mouse brain lysate using anti-Vamp8 antibody were immunoblotted with an antibody against Stx17. Ctrl, control; IP, immunoprecipitation; WB, Western blot. (C) In situ PLA between Stx17 and Vamp8 in MEFs derived from WT, AnxA2-KO, or PS1-S367A mice in complete media (Upper) or starvation media (Lower). (Scale bar, 5 μm.) (D) Quantification of number of PLA reactions from C. Note the decrease of Stx17-Vamp8 binding in the absence of either Annexin A2 or PS1 phosphorylated at Ser367 (n = 20). Data represent mean ± SEM. **P < 0.01, ***P < 0.001; t test.