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Identification of cell populations. (A) Gating strategy representative of three donors to identify MAIT, CD161–Vα7.2+, CD161–Vα7.2–, and CD161+Vα7.2– cells by flow cytometry in the immuno-proteome dataset. (B) Percent variability explained by each principal component and cumulatively. (C) Representative example from 10 donors of MR1 5-OP-RU and MR1 6-FP tetramer staining in CD161hi Vα7.2+ MAIT (red), CD161+ Vα7.2– (orange), and CD161–Vα7.2+ (blue) cells.
