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. Author manuscript; available in PMC: 2017 Jul 10.
Published in final edited form as: Bioconjug Chem. 2016 Jul 27;27(8):1830–1838. doi: 10.1021/acs.bioconjchem.6b00241

Figure 8.

Figure 8

(A) 3D printed parts for the cell culture apparatus to hold the stacks of Whatman filter paper. (B) (a) Sterilized filter papers were inoculated with BxPC-3 cells embedded in agarose and sodium alginate, (b) stacked with other filter papers, and (c) incubated together for layered cell culture. The paper stack inert holds the cells inoculated filter papers. The paper insert press allows holding the paper stack in place. The hollow tube inside the paper press insert allows addition of culture media to the growing cells without disturbing the cell layers. These inserts were contained in a chamber with the help of base and cover.