Figure 6. Impact of inactivation of pRSC on network dynamics.
(A) Left, schematic of the experiment. Right, muscimol injection sites indicated by the pink arrows, pRSC modules and directed functional connectivity from pRSC measured by Granger causality analysis.
(B) Effect of module inactivation on behavioral performance. Left, correct rate (96.2 ± 1.3 % for vehicle, 83.8 ± 6.6 % for muscimol, *p < 0.05, n = 6 mice, one-tailed bootstrap, mean ± SEM). Right, movement correlation (n.s., p = 0.19, n = 6 mice, one-tailed bootstrap, mean ± SEM).
(C) Example peri-movement activity over 1 s with vehicle or muscimol injection in pRSC (66.67 ms/image).
(D) Mean activity change with pRSC inactivation compared to vehicle (n.s. for all cortical modules, p > 0.05, n = 6 mice, one-tailed bootstrap, mean ± SEM).
(E) Mean Thalf-max in cortical modules with vehicle or muscimol injection in pRSC (n.s. for all cortical modules, p > 0.05, n = 6 mice, one-tailed bootstrap, mean ± SEM).
(F) Temporal spread of sequential activity with vehicle or muscimol injection in pRSC.
(G) Temporal spread of trial-by-trial sequential activity with vehicle or muscimol injection in pRSC n.s., p = 0.43, n = 6 mice, one-tailed bootstrap, mean ± SEM).
(H) Mean pair-wise correlations of neural trajectories in the state space defined by the 5 factors from factor analysis with vehicle or muscimol injection in pRSC (n.s., p = 0.36, n = 6 mice, one-tailed bootstrap, mean ± SEM).