Table 2. Comparison of PI3K inhibitor GI50s for control siRNA vs INPP4B siRNA-treated cell lines and parental vs CRISPR-mediated INPP4B silencing/knockout clonal cell lines.
GI50s for control siRNA vs INPP4B siRNA-treated HCC70, BT549 and MDAMB468 cell lines and GI50s for parental vs CRISPR-mediated INPP4B silencing/knockout HCC70 and BT549 clonal cell lines. GI50s were calculated with Graphpad Prism 6.0 using proliferation assay data. (pan PI3Ki: GDC-0941, PI3K-αi: BYL719, PI3K-βi: AZD6482)
| Cell Line | Knockdown Method | Condition | Pan PI3Ki GI50(μM) | PI3Kαi GI50(μM) | PI3Kβi GI50(μM) |
|---|---|---|---|---|---|
| HCC70s | siRNA | Control | 0.054 | 1.63 | 0.053 |
| INPP4B | 0.084 | 0.081 | 0.026 | ||
| CRISPR | Parental | 0.237 | 6.90 | 0.183 | |
| INPP4B | 0.093 | 2.56 | 0.296 | ||
| BT549s | siRNA | Control | 0.400 | >10 | 3.41 |
| INPP4B | 0.157 | 1.67 | 0.578 | ||
| CRISPR | Parental | 0.632 | >10 | >10 | |
| CRISPR | 0.103 | 3.26 | 2.12 | ||
| MDAMB468s | siRNA | Control | 0.101 | 3.16 | 0.131 |
| INPP4B | 0.248 | >10 | 0.041 |