Figure 2.

(A) Proliferation potential of osteoblasts (n = 6) was determined on Days 0 (seeding), 1, 10, 20, 30 and 40. Approximately one-third of seeded cells attached to the scaffold (Day 1). Osteoblasts proliferated over 40 days on scaffolds and the number of cells after 40 days was significantly higher than at Day 0 (p < 0.05) as measured in µg DNA per scaffold. A plateau-like phase was reached after 20 days; (B) Extracellular matrix deposition of osteoblasts was measured with significantly more calcium deposited at later time points than at seeding (p < 0.05). Intra- and extracellular calcium was measured in µg/scaffold and showed a linear fashion.