Figure 4.

SEM of osteoblasts cultured for 40 days in osteogenic differentiation media on melt electrospun scaffolds. (A) Overview revealed a solid layer of osteoblasts covering the entire scaffold on both the convex (B) and concave (C) side; (D) Cell detection and calcium deposition on the inside of the scaffold demonstrating infiltration of cells into the porous scaffold structure; (E,F) High resolution image of calcium deposits formed by osteoblasts on the cell layer surface. Scale bars: 2 mm (A); 25 μm (B–D); 10 μm (E); and 2 μm (F).