Figure 4.

Effects of pH and buffer composition on partitioning of proteins in the detergent and aqueous phases obtained from 4% TX-100/water two-phase separation of a membrane pre-enriched fraction of mouse myeloma cells. A and D stand for aqueous and detergent phases, respectively; pH6.8 and pH8.8 solutions are prepared by Tris buffer (0.5 M) with pH adjusted by HCl; PBS stands for phosphate buffer saline; 0.01, 0.1, 0.3, and 0.6 are the v/v fraction of 1× PBS in system.