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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Methods Mol Biol. 2017;1584:31–49. doi: 10.1007/978-1-4939-6881-7_3

Figure 1. Imaris analysis of MTOC translocation.

Figure 1

(a) Initial confocal image of T cells and APC conjugates. Z stack with staining of α-tubulin (green), F-actin (red), and CMAC for the APC (cyan). (b) Generation of the APC mask (Surface) using CMAC as reference (cyan). (c) Creation of the MTOC mask (Spots) manually selected (yellow spheres). (d) Measurement of the distance between the MTOC masks and the APC mask in a 3D system. Scale bar 5 μm