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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Methods Mol Biol. 2017;1584:31–49. doi: 10.1007/978-1-4939-6881-7_3

Figure 2. Tracking of +tips from TIRFm imaging.

Figure 2

(a) Fluorescence image from a time-lapse of an EB3-GFP expressing T cell. (b–e) Set of images from different steps of the surface generation from fluorescence time- lapse images and tracks. (b) Initial surfaces and tracks including MTOC. (c) Surfaces and track excluding MTOC. (d) Processed surfaces and tracks excluding MTOC, peripheral tracks and tracks<2 μm. (e) Tracks from (d). Bar, 2 μm. Analysis corresponds to 30 s from the acquired time-lapse. (f). Graphs showing the indi- cated information from the analysis of tracks from the cell shown in (c) excluding the MTOC. (g). Graphs show- ing the indicated information from the analysis of tracks from the cell shown in (e). Duration is expressed in s, length in μm, and speed in μm.s−1