. Author manuscript; available in PMC: 2017 Jul 10.

Published in final edited form as: ACS Chem Biol. 2016 Jun 24;11(8):2312–2319. doi: 10.1021/acschembio.6b00269

Table 1. Photophysical Properties of Multicolor DarkZone Dyes.

dye^a	λ_Abs (nm)	λ_Em (nm)	Φ_fl	e_{λ_max}	Fluorescence enhancement (fold)
BODIPY FL	507	511	0.164^b	31500	4
DEAC^c	435	477	0.009^d	29000	23
Cy3	508	559	0.001^e	53200	30
fluorescein	499	519	0.053^b	47400	12
TAMRA	556	575	0.023^e	60100	13

Measured in 300 mM phosphate buffer, pH 7.

Quantum yields measured using fluorescein standard.

7-(Diethylamino)coumarin.

Quantum yields measured using 9,10-diphenylanthracene standard.

Quantum yields measured using rhodamine B standard.