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. 2017 Jul 10;12(7):e0179696. doi: 10.1371/journal.pone.0179696

Fig 3. TNF-α is highly produced in adipose tissue of aP2-Gpr43TG mice.

Fig 3

(a) Tnfα, F4/80, and Mcp-1 mRNA expression in the epididymal adipose tissue of control WT and aP2-Gpr43TG mice fed an NC, measured using qRT-PCR (n = 6−8). (b) Epididymal adipose tissue stained with anti-F4/80 (green), anti-caveolin 1 (red) and 4’,6-diamidino-2-phenylindole (DAPI) (blue). Scale bar: 50 μm. (c) ELISA for TNF-α in the epididymal adipose tissue of control WT and aP2-Gpr43TG mice fed an NC (n = 6−8). (d) ELISA for TNF-α in the serum of control WT or aP2-Gpr43TG mice fed an NC (n = 6−8). (e) ELISA for TNF-α (upper) and Tnfα mRNA expression (lower) in the muscle (n = 6−8). (f) ELISA for TNF-α (upper) and Tnfα mRNA expression (lower) in the liver (n = 6−8). 18S mRNA expression was used as an internal control. Mice were analyzed at 16 weeks of age. All data are presented as mean ± S.E.M. *p < 0.05, **p < 0.01, N.S.: not significant.