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. 2017 Jul 10;12(7):e0179696. doi: 10.1371/journal.pone.0179696

Fig 6. Tnfα mRNA is induced in adipose tissue M2-type macrophages depending on GPR43 and SCFA stimulation.

Fig 6

(a) Gpr43 (left) and Tnfα (right) mRNA expression in epididymal adipose tissue MAs and macrophage subsets of WT mice fed an NC, measured using qRT-PCR (n = 4). 18S mRNA expression was used as an internal control. Mice were analyzed at 7−9 weeks of age. (b) Representative flow cytometric plots (left) and bar graphs summarizing the frequency and number of CD11b+F4/80+ cells by analyzing live CD45+ cells (right) extracted from the epididymal adipose tissue of WT and Gpr43-/- mice (n = 4). Mice were analyzed at 7−8 weeks of age. (c) Tnfα mRNA expression in epididymal adipose tissue derived-M2-type macrophages of WT and Gpr43-/- mice fed an NC, measured using qRT-PCR (n = 4). Mrc1 mRNA expression was used as an internal control to evaluate the Tnfα mRNA induction from a single M2-type macrophage. Mice were analyzed at 7−8 weeks of age. (d) Cecal content weights of nontreated and antibiotics-treated WT mice fed an NC (n = 5−7). Mice were analyzed at 7–10 weeks of age. (e) Quantification of short chain fatty acids in the serum of nontreated and antibiotics-treated WT mice fed an NC (n = 5−7). Mice were analyzed at 7−10 weeks of age. (f) Tnfα mRNA expression in the epididymal adipose tissue derived-M2-type macrophages of nontreated and antibiotics-treated WT and Gpr43-/- mice fed an NC, measured by qRT-PCR (n = 3−9). Mrc1 mRNA expression was used as an internal control. Mice were analyzed at 7−10 weeks of age. All data are presented as mean ± S.E.M. *p < 0.05, **p < 0.01, ***p < 0.001, N.S.: not significant.