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E-PEF experiments at room temperature. Samples were loaded with either 4 or 6%w/w enzymes and incubated for 6 h at room temperature at a pH of 7.7. The control sample was treated identically to the other samples with the exception that no proteins were added prior to the incubation. After pretreatment, samples were PEF treatment (5 pulses of 0.1 ms at 7.5 kV/cm). The data of the control experiment originates from Figure 1. Error bars represent biological replicates (n = 2).
