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. Author manuscript; available in PMC: 2017 Jul 10.
Published in final edited form as: Mol Microbiol. 2009 Nov 17;75(1):29–45. doi: 10.1111/j.1365-2958.2009.06962.x

Fig. 3.

Fig. 3

Complementation of P. aeruginosa and P. syringae transporter-deficient mutants by plasmids expressing cbcXWV from various strains.

A. Growth of PA14, PA14 containing insertions in individual cbc genes, and the PA14 mutants containing pME6041 or pME6041 expressing cbcXWV from PAO1.

B. Uptake of radiolabelled betaine, choline or carnitine (1 mM) by DC3000, B728a, B728a with insertions in the putative SBP loci Psyr_2916 (B2916Ko) and Psyr_0028 (B0028Ko), and B728a lacking opuC, betT and cbc (BT).

C. Uptake of radiolabelled choline and carnitine (1 mM) by strain BT containing the vector pME6041 or pME6041 expressing cbcXWV from DC3000, B728a or PAO1.

Cells were grown with 20 mM choline (A) or with 10 mM choline and 10 mM betaine (B and C) as sole C sources and optical density at 600 nm (OD600) was recorded after 24 h (with an initial OD600 of 0.05). Values represent the mean + SE (n = 3).