Table 1.
Strains and plasmids used in this study.
| Strain or plasmid | Description/relevant genotype | Reference or source |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| Acella | Host supporting pET15b-based expression | EdgeBio |
| MKH13 | ΔputPAΔproP2 ΔbetTIBA ΔproU::spc; Spr | Kempf and Bremer (1995) |
| Pseudomonas syringae pv. tomato | ||
| DC3000 | Wild type; RfR | Moore et al. (1989) |
| DCΔcbc | DC3000 ΔcbcXWV; Rfr | This work |
| GB1 | DC3000 ΔbetT ΔopuCA; Rfr | Chen and Beattie (2008) |
| GB2 | DC3000 ΔbetT ΔcbcXWV; Rfr | Chen and Beattie (2008) |
| GB3 | DC3000 ΔopuCA ΔcbcXWV; Rfr | Chen and Beattie (2008) |
| GB4 | DC3000 ΔbetT ΔopuCA ΔcbcXWV; Rfr | Chen and Beattie (2008) |
| Pseudomonas syringae pv. syringae | ||
| B728a | Wild type; RfR | Loper and Lindow (1987) |
| B0028Ko | B728a Psyr_0028::Tet; Rfr | This work |
| B2916Ko | B728a caiX::Tet; Rfr | This work |
| BT | B728a ΔbetT ΔopuC ΔcbcXWV; Rfr | This work |
| BT0028Ko | B728a ΔbetT ΔopuC ΔcbcXWV Psyr_0028::Tet | This work |
| BT2916Ko | B728a ΔbetT ΔopuC ΔcbcXWV caiX::Tet | This work |
| BT3758Ko | B728a ΔbetT ΔopuC ΔcbcXWV betX::Tet | This work |
| Pseudomonas aeruginosa | ||
| PA14 | Wild type | Rahme et al. (1995) |
| PA14ΔcaiX | PA14 ΔPA5388 | Wargo and Hogan (2009) |
| PA5376:tn | PA14 with transposon insertion in PA5376; Gmr | Liberati et al. (2006) |
| PA5377:tn | PA14 with transposon insertion in PA5377; Gmr | Liberati et al. (2006) |
| PA5378:tn | PA14 with transposon insertion in PA5378; Gmr | Liberati et al. (2006) |
| Plasmids | ||
| pTOK2 | Suicide vector; Tetr | Kitten and Willis (1996) |
| pTOK2T | pTOK2 with restored lacZ activity; Tetr | This work |
| pTsacB | pTOK2T with sacB; Tetr | This work |
| pT-PA5388 | pTOK2T with caiX from P. aeruginosa PAO1 | This work |
| pT-B2916 | pTOK2T with caiX from B728a | This work |
| pT-PA3236 | pTOK2T with betX from P. aeruginosa PAO1 | This work |
| pT-B3758 | pTOK2T with betX from B728a | This work |
| pT-D3758 | pTOK2T with betX from DC3000 | This work |
| pME6041 | Broad-host-range vector; Kmr | Heeb et al. (2000) |
| pCbcPAO1 | pME6041 with cbcXWV from P. aeruginosa PAO1 | This work |
| pCbcB728a | pME6041 with cbcXWV from B728a | This work |
| pCbcDC3000 | pME6041 with cbcXWV from DC3000 | This work |
| pME2916 | pME6041with caiX from B728a | This work |
| pME2915-16 | pME6041with caiX and cdhR from B728a | This work |
| pET15b | Expression vector | Novagen |
| pET-cbcB728a | pET15b with [His]6-cbcXWVB728a | This work |
| pET-cbcDC3000 | pET15b with [His]6-cbcXWVDC3000 | This work |
| pN | pME6041 with nptII promoter | This work |
| pNcbcWV | pN with cbcWV from P. aeruginosa PAO1 | This work |
| pNcbcXWV | pN with cbcXWV from P. aeruginosa PAO1 | This work |
| pNchoXWV | pN with choXWV from S. meliloti 1021 | This work |