Table 2.
Uptake activities of E. coli strains expressing cbcXWV from various Pseudomonas spp.
| Strainb | Plasmid | Total uptake [nmol (mg protein)−1]a
|
||
|---|---|---|---|---|
| Choline | Betaine | Carnitine | ||
| MKH13 | pME6041 | 0.2 (0.1) | 0.5 (0.5) | 0.5 (0.3) |
| MKH13 | pCbcPAO1 | 12.1 (1.1) | 5.8 (1.1) | 0.8 (0.4) |
| Acellac | pET15b | 0 | 8.2 | 0 |
| Acella | pET-cbcDC3000 | 15.0 (2.4) | 17.0 (1.8) | 0.59 (0.82) |
| Acellac | pET-cbcB728a | 19.2 | 16.2 | 0 |
a
Uptake was evaluated using radiolabelled choline, betaine or carnitine (1 mM). Unless otherwise indicated, average values (SE) are shown (n = 10).
b
The Acella cells were grown with 1 mM IPTG for promoter induction.
c
Uptake values for these constructs represent a single colony; these values were verified in a separate experiment.